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TranslatorX server
Nucleotide sequence alignment and alignment cleaning based on amino acid information.
Powered by ReadSeq, GBlocks, Jalview, Muscle, Clustalw, MAFFT, T-Coffee and PRank
Download local version of TranslatorX
         
Nucleotide sequences
Paste your nt-sequences below (most formats are accepted by ReadSeq)
Example (Mollusca CytB genes)


or upload a file:
Protein alignment. Two alternatives are available:
A. Let TranslatorX automatically compute the protein alignment.
Which method do you prefer?
Muscle
MAFFT
ClustalW
T-coffee
Prank
B. Provide your own alignment.
Paste the protein alignment below

or upload a file:

Genetic code
A. Same for all taxa
Translate according to the genetic code
B.1. Variable. Define it interactively.
The most common code in my dataset is the genetic code.
B.2. Variable. A priori definition.
Paste a list of "Taxon_name Genetic_code_Index" (tab or comma separated)

or upload a file:
1 - Standard
2 - Vertebrate Mitochondrial
3 - Yeast Mitochondrial
4 - Mold Mitochondrial; ...
5 - Invertebrate Mitocho...
6 - Ciliate Nuclear; Das...
9 - Echinoderm Mitochond...
10 - Euplotid Nuclear
11 - Bacterial and Plant...
12 - Alternative Yeast N...
13 - Ascidian Mitochondr...
14 - Alternative Flatwor...
15 - Blepharisma Macronu...
16 - Chlorophycean Mitoc...
21 - Trematode Mitochond...
22 - Scenedesmus obliquu...
23 - Thraustochytrium Mi...
100 - Ancestral Arthropo...
101 - Hemichordate Mitoc...

Guess most likely reading frame? If you are not sure if codons start at first, second or third base, TranslatorX determines the most likely reading frame (that with the least number of stop codons)
If you are not sure if codons start at first, second or third base, TranslatorX can determine the most likely reading frame (that with the least number of stop codons).
Alignment cleaning
Remove poorly aligned sites from the protein alignment before back-translate to nucleotides?
GBlocks parameters
Options for a less stringent selection:
Allow smaller final blocks
Allow gap positions within the final blocks
Allow less strict flanking positions

Options for a more stringent selection:
Do not allow many contiguous nonconserved positions

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Citation: Abascal F, Zardoya R, Telford MJ (2010)
TranslatorX: multiple alignment of nucleotide sequences guided by amino acid translations
Nucleic Acids Res. 38:W7-13.
visitors since 15th Dec 2009